ASCP : ASCP-MLT Questions & Answers
Last Updated: Oct 01, 2026
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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Hematology | 20-25% | - Red blood cell physiology and disorders - White blood cell morphology and diseases - Hemostasis and coagulation testing - Routine and automated hematology procedures |
| Clinical Chemistry | 20-25% | - Carbohydrates, lipids, proteins, and enzymes - Endocrinology and therapeutic drug monitoring - Toxicology and liver/kidney function tests - Electrolytes, acid-base balance, and blood gases |
| Urinalysis and Other Body Fluids | 5-10% | - Cerebrospinal, serous, and synovial fluids - Semen and amniotic fluid testing - Urine formation and composition - Physical, chemical, and microscopic analysis |
| Laboratory Operations | 5-10% | - Laboratory safety and regulatory compliance - Laboratory mathematics and instrumentation - Pre-analytical, analytical, and post-analytical processes - Quality control and quality assurance |
| Immunology and Serology | 5-10% | - Infectious disease serology - Immunologic testing methodologies - Immune system principles and components - Autoimmune and immunodeficiency disorders |
| Microbiology | 15-20% | - Mycology, parasitology, and virology - Specimen collection and culture techniques - Identification of pathogenic microorganisms - Bacteriology and antimicrobial susceptibility |
| Blood Banking (Immunohematology) | 15-20% | - Transfusion reactions and adverse events - Blood group systems and antigens - Antibody detection and identification - Blood components and storage - Compatibility testing |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Know what you are handling - read label carefully.
What is the first thing you do before you handle or open a chemical:
- A. Consult procedure manual for information
- B. Ask a supervisor for assistance
- C. Obtain a copy of the SDS
- D. Read the label
Correct Answer: D 🗳️
The role of the laboratory supervisor or manager is to
- A. Screen cytology for Pap smears.
- B. Supervise technical aspects of testing.
- C. Supervise business functions of testing.
- D. Examine surgically removed organ
Correct Answer: C 🗳️
The production of long, tapered phialides is one of the key identifying features of Trichoderma species. In contrast, Penicillium species, produce phialides with blunt ends.
The phialides of Beauveria species are geniculate, forming a zig-zag pattern.
The arrows in the image on the right point to single, long, tapered phialides that extend laterally from either side of the hyphae. This is an identifying feature of which of the fungi listed below?
- A. Beauveria species
- B. Trichoderma species
- C. Penicillium species
Correct Answer: B 🗳️
The end of the initial phase of HH treatment is considered when the serum ferritin decreases to between 20 and
50 ng/mL.
What is a typical finding for determining the endpoint for the initial or iron-depletion phase of treatment for hereditary hemochromatosis (HH)?
- A. The transferrin saturation drops below 20%
- B. The serum iron falls to below 35 g/dL.
- C. The serum ferritin decreases to between 20 and 50 ng/mL
- D. The hepatic iron index returns to normal
Correct Answer: C 🗳️
Urinalysis & Other Body Fluids
Match the following urine chemical reagent strip test pads to the disease or disorder that would most likely cause a positive test result.
1. Ketones
2. Blood
3. Bilirubin
4. Nitrites
- A. Renal calculi
- B. Diabetes mellitus
- C. Hepatitis/cirrhosis
- D. Urinary tract infection
Correct Answer: A,B,C,D 🗳️
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